Leishmania IgG / IgM Ikizamini cya Antibody Ikizamini (Immunochromatographic Assay)

Ibisobanuro bigufi:

Ingero	Serumu / Plasma / Amaraso Yose	Imiterere	Cassette
Ibyiyumvo	98.83%	Umwihariko	98.02%
Trans.& Sto.Ubushuhe.	2-30 ℃ / 36-86 ℉	Igihe cyo Kugerageza	Iminota 5-10
Ibisobanuro	1 Ikizamini / Kit 5 Ibizamini / Kit 25 Ibizamini / Kit

Ohereza imeri kuri twe

Ibicuruzwa birambuye

Ibicuruzwa

Ibisobanuro birambuye

Gukoresha
Iki gicuruzwa kibereye kwisuzumisha ryujuje ubuziranenge bwa serumu / plasma / icyitegererezo cyamaraso kugirango hamenyekane antibodiyite zirwanya Leishmania.Nibizamini byoroshye, byihuse kandi bidafite ibikoresho byo gusuzuma kala-azar yatewe na Leishmania.

Ihame ry'ikizamini
Ibicuruzwa ni urujya n'uruza rwa chromatografique immunoassay.Cassette yikizamini igizwe na: 1) padi yamabara ya burgundy irimo recombinant rK39 antigen ihujwe na zahabu ya colloid (Leishmania conjugates) hamwe ninkwavu IgG-zahabu;2) agace ka nitrocellulose karimo ibice bibiri byipimisha (M na G band) hamwe nigenzura (C band).

Ibirimo

Ibice byatanzwe biri kurutonde.

Ibikoresho / byatanzwe	Umubare (1 Ikizamini / Kit)	Umubare (5Ibizamini / Kit)	Umubare (25Ibizamini / Kit)
Ikizamini	Ikizamini 1	Ibizamini 5	Ibizamini 25
Buffer	Icupa 1	Amacupa 5	25/2 amacupa
Igitonyanga	Igice 1	5 pc	25 pc
Isakoshi yo gutwara abantu	Igice 1	5 pc	25 pc
Ikoreshwa rya Lancet	Igice 1	5 pc	25 pc
Amabwiriza yo Gukoresha	Igice 1	Igice 1	Igice 1
Icyemezo cyo guhuza	Igice 1	Igice 1	Igice 1

Ibikorwa

Intambwe ya 1: Gutoranya

Kusanya Serumu yumuntu / Plasma / Amaraso yose uko yakabaye.

Intambwe ya 2: Kwipimisha

Nyamuneka soma amabwiriza witonze mbere yo kwipimisha.Mbere yo kwipimisha, emera ibikoresho byikizamini, igisubizo cyicyitegererezo hamwe nicyitegererezo kuringaniza ubushyuhe (15-30 ℃ cyangwa dogere 59-86 Fahrenheit).
1.Kuraho umuyoboro ukuramo mugikoresho hamwe nagasanduku k'ibizamini mu gikapu cya firime ushwanyaguza.Shyira ku ndege itambitse.
2.Fungura ikarita yo kugenzura aluminium foil umufuka.Kuraho ikarita yikizamini hanyuma uyishyire kuri horizontalale kumeza.
3.Koresha umuyoboro wajugunywe, ohereza igitonyanga kimwe (hafi 20μL) amaraso yintoki / cyangwa 4μL serumu / cyangwa 4μL plasma / cyangwa 4μL maraso yose mumashusho neza kuri cassette yikizamini.
4.Fungura umuyoboro wa buffer.Shira ibitonyanga 3 (hafi 80 μL) bya assay diluent muri assay diluent neza neza.Tangira kubara.

Intambwe ya 3: Gusoma

Soma ibisubizo kuminota 5-10.Ibisubizo nyuma yiminota 10 ntabwo byemewe.

Ibisobanuro

Ibisubizo bibi
Niba gusa umurongo wo kugenzura ubuziranenge C ugaragara kandi imirongo yo gutahura G na M ntabwo yerekana

Igisubizo cyiza
1.Bombi umurongo wo kugenzura ubuziranenge C n'umurongo wo gutahura M ugaragara = antibody ya Leishmania IgM iramenyekana, kandi ibisubizo nibyiza kuri antibody ya IgM.
2.Bombi kumurongo wo kugenzura ubuziranenge C n'umurongo wo gutahura G ugaragara = antibody ya Leishmania IgG iramenyekana kandi ibisubizo nibyiza kuri antibody ya IgG.
3.Byombi umurongo ugenzura ubuziranenge C hamwe numurongo wo gutahura G na M ugaragara = antibodiya ya Leishmania IgG na IgM.ziramenyekana, kandi ibisubizo nibyiza kuri antibodies zombi za IgG na IgM.

Ibisubizo bitemewe
Umurongo wo kugenzura ubuziranenge C ntushobora kugaragara, ibisubizo bizaba impfabusa utitaye ko umurongo wikizamini werekana, kandi ikizamini kigomba gusubirwamo.

Tegeka amakuru

izina RY'IGICURUZWA	Injangwe.Oya	Ingano	Ingero	Ubuzima bwa Shelf	Trans.& Sto.Ubushuhe.
Leishmania IgG / IgM Ikizamini cya Antibody Ikizamini (Immunochromatographic Assay)	B020C-01	1test / kit	Serumu / Plasma / Amaraso Yose	Amezi 18	2-30 ℃ / 36-86 ℉
	B020C-05	Ibizamini 5
	B020C-25	Ibizamini 25