Tifoyide IgG / IgM Ikizamini cya Antibody (Immunochromatographic Assay)
Ibisobanuro birambuye
Gukoresha
Tifoyide IgG / IgM Antibody Test Kit (Immunochromatographic Assay) ikoresha uburyo bwa zahabu ya colloidal kugirango ibone neza antibody ya tifoyide bacillus (lipopolysaccharide antigen na membrane membrane protein antigen) muri serumu / plasma yumuntu, ikwiranye no gusuzuma hakiri kare infashanyo ya tifoyide. .
Ihame ry'ikizamini
Tifoyide IgG / IgM Ikizamini cya Antibody Ikizamini (Immunochromatographic Assay) ni urujya n'uruza rwa chromatografiya immunoassay.Cassette yipimisha igizwe na: 1) padi ya conjugate yamabara ya burgundy irimo recombinant S. typhoid H antigen na O antigen yahujwe na zahabu ya colloid (Tifoyide conjugates) hamwe ninkwavu IgG ‐gold conjugate, 2) agace ka nitrocellulose karimo ibice bibiri byipimisha (IgG na IgM band) hamwe nitsinda rishinzwe kugenzura (C band).Itsinda rya IgM ryateguwe mbere na monoclonal anti-muntu IgM kugirango hamenyekane IgM anti-S.typhi, itsinda rya IgG ryashizwemo mbere na reagent kugirango hamenyekane IgG anti-S.typhi, naho C itsinda ryabanje gushyirwaho ihene irwanya urukwavu IgG.
Ibirimo
Ibice byatanzwe biri kurutonde.
| Ibikoresho / byatanzwe | Umubare (1 Ikizamini / Kit)
| Umubare (5Ibizamini / Kit)
| Umubare (25Ibizamini / Kit)
|
| Ikizamini | Ikizamini 1 | Ibizamini 5 | Ibizamini 25 |
| Buffer | Icupa 1 | Amacupa 5 | 25/2 amacupa |
| Igitonyanga | Igice 1 | Igice 5 | Igice 25 |
| Isakoshi yo gutwara abantu | Igice 1 | 5 pc | 25 pc |
| Ikoreshwa rya Lancet | Igice 1 | 5 pc | 25 pc |
| Amabwiriza yo Gukoresha | Igice 1 | Igice 1 | Igice 1 |
| Icyemezo cyo guhuza | Igice 1 | Igice 1 | Igice 1 |
Ibikorwa
Intambwe ya 1: Gutoranya
Kusanya Serumu yumuntu / Plasma / Amaraso yose uko yakabaye.
Intambwe ya 2: Kwipimisha
1. Kuraho umuyoboro ukuramo mugikoresho hamwe nagasanduku k'ibizamini mu gikapu cya firime ushishimura.Fungura ikarita yo kugenzura aluminium foil umufuka.Kuraho ikarita yikizamini hanyuma uyishyire utambitse kumeza.
2. Koresha imiyoboro ikoreshwa, ohereza serumu 4μL (cyangwa plasma), cyangwa 4μL maraso yose muri sample neza kuri cassette yipimisha.
3.Fungura umuyoboro wa buffer uhinduranya hejuru.Shira ibitonyanga 3 (hafi 80 μL) bya assay diluent muri assay diluent neza neza.Tangira kubara.
Intambwe ya 3: Gusoma
Soma ibisubizo saa 15 min.Ibisubizo nyuma yiminota 20 ntabwo byemewe.
Ibisobanuro
Ibisubizo bibi
Gusa umurongo wo kugenzura ubuziranenge C ugaragara kandi imirongo yo gutahura G na M ntabwo yerekana, bivuze ko nta antibody ya Tifoyide yagaragaye kandi ibisubizo ni bibi.
Igisubizo cyiza
Niba byombi umurongo ugenzura ubuziranenge C n'umurongo wo gutahura M ugaragara = antibody ya Tifoyide IgM yamenyekanye, kandi ibisubizo nibyiza kuri antibody ya IgM.
Niba byombi umurongo wo kugenzura ubuziranenge C n'umurongo wo gutahura G ugaragara = antibody ya Tifoyide IgG iragaragara kandi ibisubizo nibyiza kuri antibody ya IgG.
Niba byombi umurongo wo kugenzura ubuziranenge C hamwe numurongo wo gutahura G na M ugaragara = antibodiyite ya Tifoyide IgG na IgM iramenyekana, kandi ibisubizo nibyiza kuri antibodiya zombi za IgG na IgM.
Ibisubizo bitemewe
Niba umurongo wo kugenzura ubuziranenge C udashobora kugaragara, ibisubizo bizaba bitemewe
utitaye ko umurongo wikizamini werekana, kandi ikizamini kigomba gusubirwamo.
Tegeka amakuru
| izina RY'IGICURUZWA | Injangwe.Oya | Ingano | Ingero | Ubuzima bwa Shelf | Trans.& Sto.Ubushuhe. |
| Tifoyide IgG / IgM Ikizamini cya Antibody (Immunochromatographic Assay) | B023C-01 | 1test / kit | Serumu / Plasma / Amaraso Yose | Amezi 18 | 2-30 ℃ / 36-86 ℉ |
| B023C-05 | Ibizamini 5 | ||||
| B023C-25 | Ibizamini 25 |
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