SARS-CoV-2 Kutabogama ibikoresho byo gupima antibody (Chromatografiya)

Gukoresha

SARS-CoV-2 Kutabogama kwa antibody Ikizamini (Lateral chromatografiya) irakwiriye muri vitro yujuje ubuziranenge bwihuse bwo kumenya SARS-CoV-2 itabuza antibodiyite muri serumu uman, plasma, cyangwa amaraso yose (capillary cyangwa venine).Igikoresho kigenewe nk'imfashanyo yo gusuzuma imiterere irwanya imiterere ya SARS-CoV-2.Kuri vitro kwisuzumisha koresha gusa.Gukoresha umwuga gusa. 

Ihame ry'ikizamini

SARS-CoV-2 Kutabogama kwa antibody Ikizamini (Lateral chromatografiya) ni immunoassay ishingiye kuri membrane yujuje ubuziranenge kugirango hamenyekane antibodiyite za SARS-CoV-2 RBD muri serumu, plasma n'amaraso yose.Icyitegererezo cyamanuwe muri sample neza kandi sample ya dilution buffer yongeweho nyuma.Antibodiyite za SARS-CoV-2 RBD murugero zifatanije nuduce twanditseho proteine ​​ya RBD hanyuma tugakora urwego rwumubiri.Mugihe uruganda rwimuka kuri nitrocellulose ya membrane kubikorwa bya capillary, antibodiyite za RBD zirashobora gufatwa nindi proteine ​​ya RBD yashizwe kumwanya wikizamini (T umurongo), ikora umurongo wikimenyetso.Agace kagenzura ubuziranenge gashizwemo ihene irwanya inkoko IgY, kandi inkoko igabanijwe IgY ifatwa kugirango ibe igoye kandi yegeranye mu murongo wa C.Niba umurongo C utagaragara, byerekana ko ibisubizo bitemewe, kandi gusubiramo birakenewe.