Recombinant Monkeypox Virus B6R Poroteyine, C-Ikimenyetso cye

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Ibicuruzwa birambuye

Inkomoko	Virusi ya Monkeypox (shyira Zaire-96-I-16)
Ikirangantego	HEK 293 Utugari
Tag	C-Ikimenyetso cye
Gusaba	Birakwiye gukoreshwa muri immunoassays. Buri laboratoire igomba kumenya titer nziza yo gukora kugirango ikoreshwe mubikorwa byayo.
Amakuru rusange	Indwara ya Recombinant monkeypox virusi B6R ikorwa na sisitemu ya Mammalianexpression kandi gene igenewe kodegisi Tyr18-Thr277 igaragazwa na tagi ye kuri C-terminus.

Isuku	> 95% nkuko byagenwe na SDS-PAGE.
Misa ya molekulari	Poroteyine ya Recombinant Monkeypox Virus B6R igizwe na aside amine 275 kandi ifite misile ibarwa ya 30.5 kDa.Poroteyine yimuka nka 40.0 kDa mugabanya SDS-PAGE kubera glycosylation.
Buffer	10 MM PB, 300 mM NaCl, pH 8.0.
Ububiko	Ubike mubihe bidasanzwe kuri -20 ℃ kugeza -80 ℃ umaze kwakira. Saba kugabanya poroteyine muke kugirango ubike neza.