Recombinant Monkeypox Virus A35R Poroteyine, C-Ikimenyetso cye

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Ibicuruzwa birambuye

Inkomoko	Virusi ya Monkeypox (shyira Zaire-96-I-16)
Ikirangantego	HEK 293 Utugari
Tag	C-Ikimenyetso cye
Gusaba	Birakwiye gukoreshwa muri immunoassays. Buri laboratoire igomba kumenya titer nziza yo gukora kugirango ikoreshwe mubikorwa byayo.
Amakuru rusange	Indwara ya Recombinant monkeypox virusi A35R ikorwa na sisitemu yo kwerekana inyamaswa z’inyamabere kandi intego ya gene irimo Arg58-Thr181 igaragazwa na tagi ye kuri C-terminus.

Isuku	> 95% nkuko byagenwe na SDS-PAGE.
Misa ya molekulari	Virusi ya recombinant monkeypox A35R proteine igizwe na acide amine 139 kandi ifite misile ibarwa ya 15.3 kDa.Poroteyine yimuka nka 15-26 kDa mugabanya SDS-PAGE kubera glycosylation.
Buffer	20 mM Tris, 10 mM NaCl, pH 8.0.
Ububiko	Ubike mubihe bidasanzwe kuri -20 ℃ kugeza -80 ℃ umaze kwakira. Saba kugabanya poroteyine muke kugirango ubike neza.