Prokaryotic Protein Kugaragaza
Sisitemu ya imvugo ya prokaryyotike E. coli izwi cyane nkigiciro cyinshi cyane, gikuze mubuhanga, kandi gikunze gukoreshwa muburyo bwo kwerekana poroteyine.Kuri Bioantibody, twiyemeje guha abakiriya bacu serivisi zuzuye, zihagarara rimwe uhereye kuri synthesis ya gene kugeza imvugo ya protein no kwezwa.Serivisi zacu zirimo kodegisi yubusa no gukoresha tekinoroji yacu yihariye kugirango dukemure ibibazo byose bijyanye no kuvuga nabi no kudashobora guhinduka bishobora kuvuka mugihe cyose cyo kuvuga no kweza.Abakiriya bacu bakeneye gusa gutanga gene cyangwa aminide acide ya poroteyine kandi dushobora gutanga poroteyine nziza cyane nka FAST nkibyumweru bitatu.Byongeye kandi, Bioantibody itanga endotoxin yo gukuraho no gutondeka serivisi zijyanye nibyo abakiriya bacu bakeneye.Twiyemeje gutanga ibisubizo kandi dusezeranya ko tutazishyuza amafaranga iyo poroteyine yanyuma itagaragaye.
Ibintu bya serivisi | Ibirimo Ubushakashatsi | Kuyobora Igihe (BD) |
Gene Synthesis | Kunonosora Codon, synthesis ya gene na subcloning. | 5-10 |
Kugaragaza Kumenyekanisha no Gukemura Isesengura | 1. Guhinduka hamwe na incubation, kwerekana imvugo hamwe na SDS-PAGE.2. Isesengura ryibisubizo, SDS-PAGE hamwe no kumenya WB | 10 |
Inkubasi nini no kwezwa, proteine yanyuma (ubuziranenge> 85%, 90%, 95%) na raporo yubushakashatsi busanzwe | Isuku ya Affinity (Ni inkingi, MBP, GST) |
Niba gene ikomatanyirijwe muriBioantibody, plasmid yubatswe izashyirwa mubitangwa.