H. pylori Intebe Antigen Ikizamini cyihuse

H. pylori Intebe Antigen Yihuta,
H. Pylori Antigen, H. pylori baterial, H. pylori nziza, Ikizamini cya Pylori, helicobacter pylori faecal antigen,
Gukoresha

H. Pylori AntigenIkizamini cyihuta (Chromatography Lateral) kigomba gukoreshwa muri vitro yujuje ubuziranenge bwa helicobacter pylori antigen mumyanda yabantu.Ikizamini kigomba gukoreshwa gusa nabashinzwe ubuvuzi.

Ihame ry'ikizamini

Igikoresho ni immunochromatographic kandi ikoresha uburyo bwa kabiri-antibody sandwich kugirango imenyeH. Pylori Antigen.Harimo ibara ryamabara ya spherical yanditseho antibody ya H. Pylori monoclonal ipfunyitse muri padi ya conjugate.Indi antibody ya H. Pylori monoclonal yashyizwe kuri membrane ya NC.n'umurongo wo kugenzura ubuziranenge C ushyizwemo na antibody y'ihene irwanya imbeba IgG, ukoreshe antigen-antibody yihariye hamwe na tekinoroji ya chromatografiya, ugena neza urwego rwa H. Pylori Antigen mumyanda yabantu.

Ibirimo

Ibice byatanzwe biri kurutonde.

Ibikorwa

Intambwe ya 1: Gutoranya

Kusanya fecalspecimensin isukuye, idashobora kumeneka.

Intambwe ya 2: Kwipimisha

1.Kuraho cassette yikizamini mumufuka wa file hanyuma ushire hejuru

2.Kuramo icupa ry'icyitegererezo, koresha inkoni isaba yometse kumutwe kugirango wimure agace gato k'icyitegererezo cy'intebe (mm 3-5 z'umurambararo; hafi 30-50 mg) mumacupa y'icyitegererezo irimo buffer yo gutegura.

3.Simbuza inkoni mu icupa hanyuma ukomere neza.Kuvanga icyitegererezo cyintebe hamwe na buffer neza uzunguza icupa inshuro nyinshi hanyuma usige umuyoboro wenyine muminota 2.

4. Kuramo icupa ry'icyitegererezo hanyuma ufate icupa mumwanya uhagaze hejuru y'iriba ry'icyitegererezo cya Cassette, utange ibitonyanga 3 (hafi 100 -120μL) by'icyitegererezo cy'intebe ku cyitegererezo.

Intambwe ya 3: Gusoma

Nyuma yiminota 15, soma ibisubizo mumashusho.(Icyitonderwa: Ntugasome ibisubizo nyuma yiminota 20!)

Ibisobanuro

1.Ibisubizo bibi

Niba gusa umurongo wo kugenzura ubuziranenge C ugaragara kandi imirongo yo gutahura G na M itagaragaza, bivuze ko nta antibody ya coronavirus yamenyekanye kandi ibisubizo ni bibi.

2. Igisubizo cyiza

2.1 Niba umurongo ugenzura ubuziranenge C n'umurongo wo gutahura M ugaragara, bivuze ko igitabo gishya cya coronavirus IgM antibody cyamenyekanye, kandi ibisubizo ni byiza kuri antibody ya IgM.

2.2 Niba umurongo ugenzura ubuziranenge C n'umurongo wo gutahura G ugaragara, bivuze ko igitabo gishya cya coronavirus IgG antibody cyamenyekanye kandi ibisubizo ni byiza kuri antibody ya IgG.

2.3 Niba umurongo ugenzura ubuziranenge C hamwe numurongo wo gutahura G na M ugaragara, bivuze ko habonetse antibodiyite coronavirus IgG na IgM, kandi ibisubizo nibyiza kuri antibodi zombi za IgG na IgM.

3. Ibisubizo bitemewe

Niba umurongo ugenzura ubuziranenge C udashobora kugaragara, ibisubizo bizaba impfabusa utitaye ko umurongo wikizamini werekana, kandi ikizamini kigomba gusubirwamo.

Tegeka amakuru

H. pylori ni bagiteri ishobora kwanduza igifu.Ni imwe mu mpamvu zikunze gutera indwara ya gastrite n'indwara ya peptike.

Ikizamini cya H. pylori antigen igaragaza antibodies kuri bagiteri mumaraso yawe.Niba waranduye H. pylori, izo antibodies zizaba ziri mumaraso yawe.