Cardiac Troponin I Ikizamini Cyihuta (Chromatografiya)
Ibisobanuro birambuye
Gukoresha Intego:
Cardiac Troponin I Rapid Test Kit ikoresha ikoreshwa rya zahabu ya immunochromatografiya kugirango tumenye umutima Troponine I (cTnI) muri serumu, plasma cyangwa icyitegererezo cyamaraso yose yujuje ubuziranenge cyangwa igice cya kabiri hamwe namakarita asanzwe ya colimetric.Iki kizamini gikoreshwa nkubufasha mugupima imvune ya myocardial nka Acute Myocardial Infarction, Angina idahindagurika, Acute Myocarditis na Syndrome ya Acute Coronary.
Amahame y'Ikizamini:
Cardiac Troponin I Rapid Test Kit (Lateral Chromatography) ni ireme cyangwa igice cyuzuye, membrane ishingiye kuri immunoassay kugirango hamenyekane umutima Troponine I (cTnI) mumaraso yose, serumu cyangwa plasma.Muri ubu buryo bwo gukora ibizamini, gufata reagent birahagarikwa mumurongo wikizamini cyikizamini.Nyuma yikigereranyo cyongewe kumwanya wikigereranyo cya cassette, ikora hamwe na antibody anti-cTnI yatewe mubice.Iyi mvange yimuka chromatografique muburebure bwikizamini kandi ikorana na reagent ya immobilized.Imiterere yikizamini irashobora kumenya umutima Troponin I (cTnI) mubigereranyo.Niba icyitegererezo kirimo umutima wa Troponine I (cTnI), umurongo wamabara uzagaragara mukarere kizamini kandi ubukana bwamabara bwumurongo wikizamini bwiyongera ugereranije nubushakashatsi bwa cTnI, byerekana ibisubizo byiza.Niba icyitegererezo kitarimo umutima wa Troponine I (cTnI), umurongo wamabara ntuzagaragara muri kano karere, byerekana ingaruka mbi.Kugirango ube igenzura ryikurikiranabikorwa, umurongo wamabara uzahora ugaragara mukarere kayobora umurongo, byerekana ko ingano yikigereranyo yongeweho kandi ibyabaye byabayeho.
Ibirimo
Ibice byatanzwe biri kurutonde.
| Ibigize REF REF | B032C-01 | B032C-25 |
| Ikizamini | Ikizamini 1 | Ibizamini 25 |
| Icyitegererezo | Icupa 1 | Icupa 1 |
| Igitonyanga | Igice 1 | 25 pc |
| Ikarita isanzwe yamabara | Igice 1 | Igice 1 |
| Icyemezo cyo guhuza | Igice 1 | Igice 1 |
Ibikorwa
Intambwe ya 1: Icyitegererezo
1. Ibikoresho byo kwipimisha birashobora gukorwa hakoreshejwe amaraso yose, serumu cyangwa plasma.Tanga igitekerezo cyo guhitamo serumu cyangwa plasma nkicyitegererezo.Niba uhisemo amaraso yose nkicyitegererezo, igomba gukoreshwa hamwe namaraso ya diluent.
2. Gerageza icyitegererezo ku ikarita yikizamini ako kanya.Niba kwipimisha bidashobora kurangira ako kanya, icyitegererezo cya serumu na plasma bigomba kubikwa kugeza kumunsi 7 kuri 2 ~ 8 ℃ cyangwa kubikwa kuri -20 ℃ amezi 6 (icyitegererezo cyamaraso cyose kigomba kubikwa kugeza kumunsi 3 kuri 2 ~ 8 ℃ ) kugeza igihe ishobora kugeragezwa.
3. Ingero zigomba gusubizwa ubushyuhe bwicyumba mbere yo kwipimisha.Ingero zikonje zirasabwa gukonjeshwa burundu no kuvangwa neza mbere yo kwipimisha, birinda gukonjeshwa inshuro nyinshi.
4. Irinde gushyushya ingero, zishobora gutera hemolysis hamwe na protein.Birasabwa kwirinda gukoresha urugero rwinshi rwa hemolyzed.Niba icyitegererezo gisa nkicyuma gikabije, ikindi cyitegererezo kigomba kuboneka no kugeragezwa.
Intambwe ya 2: Kwipimisha
1. Nyamuneka soma igitabo witonze mbere yo kwipimisha, kugarura icyitegererezo, ikarita yipimisha hamwe nicyitegererezo cyamaraso cyoroshye kubushyuhe bwicyumba hanyuma ubare ikarita.Tanga igitekerezo cyo gufungura igikapu nyuma yo gukira ubushyuhe bwicyumba no gukoresha ikarita yikizamini ako kanya.
2. Shira ikarita yikizamini kumeza isukuye, ushyizwe muburyo butambitse.
Kuri Serumu cyangwa Plasma urugero:
Fata igitonyanga gihagaritse hanyuma wohereze ibitonyanga 3 bya serumu cyangwa plasma (hafi 80 L, Pipette irashobora gukoreshwa mugihe cyihutirwa) kurugero rwiza, hanyuma utangire igihe.Reba ingero zikurikira.
Kumaraso Yuzuye:
Fata igitonyanga gihagaritse hanyuma wohereze ibitonyanga 3 byamaraso yose (hafi 80 L) kurugero rwiza, hanyuma ongeramo igitonyanga 1 cya Sample diluent (hafi 40 L), hanyuma utangire igihe.Reba ingero zikurikira.
Intambwe ya 3: Gusoma
Mu minota 10 ~ 30, shakisha igisubizo cya kimwe cya kabiri ukurikije ikarita isanzwe ya colimetric ukoresheje amaso.
Gusobanura ibisubizo
Byemewe: Umurongo umwe utukura ugaragara kumurongo ugenzura (C).Kubijyanye nibisubizo byemewe, urashobora kubona kimwe cya kabiri cyumubare wamaso hamwe namakarita asanzwe ya colorimetric:
Ibara ryinshi vs Ibitekerezo
| Ubwinshi bwamabara | Kwibanda kuri (ng / ml) |
| - | ＜0.5 |
| + - | 0.5 ~ 1 |
| + | 1 ~ 5 |
| + | 5 ~ 15 |
| + + | 15 ~ 30 |
| + + + | 30 ~ 50 |
| + + + | ＞50 |
Ntibyemewe: Nta murongo utukura wijimye ugaragara kumurongo wigenzura (C) .Ibyo bivuze ko ibikorwa bimwe bigomba kuba bibi cyangwa ikarita yikizamini yamaze kuba impfabusa.Kuri ibi bihe, nyamuneka ongera usome igitabo witonze, hanyuma wongere ugerageze ukoresheje cassette nshya yikizamini.Niba ikibazo nk'iki cyongeye kubaho, ugomba guhagarika gukoresha ako kanya ibicuruzwa hanyuma ukabaza uwaguhaye isoko.
Tegeka amakuru
| izina RY'IGICURUZWA | Injangwe.Oya | Ingano | Ingero | Ubuzima bwa Shelf | Trans.& Sto.Ubushuhe. |
| Cardiac Troponin I Ikizamini Cyihuta (Chromatografiya) | B032C-01 | Ikizamini / kit | S / P / WB | Amezi 24 | 2-30 ℃ |
| B032C-25 | Ibizamini 25 |